Stable Isotope Peptides
Advanced Peptides specialises in the rapid synthesis of peptides containing stable isotope labelled amino acids.
By using stable-labelled versions of tryptic peptides as internal standards, it is possible to directly quantify protein levels in a digested sample via mass spectrometry.
Critical to the accuracy of this technique is the quality of the stable-labelled peptide. CRB’s custom stable–labelled peptides are offered:
- Critical to the accuracy of this technique is the quality of the stable-labelled peptide.
- >95% pure by HPLC
- analysed for net peptide content.
For the quantitation of post-translational modifications, CRB can also offer stable-labelled peptides containing:
- phosphoTyr, phosphoSer, phosphoThr
- methylated Lys and Arg
Isotopically labelled versions of peptides derived from proteolysis by other enzymes, such as Lys-C, Glu-C, can also be produced.
Our standard stable-isotopically labelled amino acids provide the greatest shift in the molecular weight for the least cost; however, heavy atom versions of other amino acids are available upon request.