FAQ - Advanced Peptides

General Info

Custom Peptide Design and Production

Peptide Chemistry

How do I place my order?

Place your order by emailing sales@advancedpeptides.com.

How do I check the status of my order?

To check the status of your order, please email sales@advancedpeptides.com.

What if I need a common peptide available in most catalogs?

Advanced Peptides supplies bulk peptides for retail. If you're interested in a particular peptide, please contact us for availability.

Can you make cGMP peptides?

Advanced Peptides specializes in the production of cGMP peptides, meeting stringent regulatory standards for clinical trials and drug production. Our dedicated cGMP facility is equipped to handle complex projects with precision.

Can I have special vialing?

Our specialized vialing services provide tailored packaging solutions, ensuring your peptides are delivered in pre-determined, user-friendly quantities, ready for immediate application in your research or distribution.

What if I need to make peptide-based antibodies?

Peptides are commonly used for antibody production. If you require assistance with antibody production, we can provide recommendations for specialized labs.

What do I do with the C-terminus and N-terminus of my peptide?

Most biologically active custom peptides mimic proteins or cleaved protein products with naturally occurring unprotected termini. If the sequence is not a known cleavage product, blocking the termini might be necessary. This decision depends on the specific sequence and intended use.

What purity should I choose for my research?

The choice of purity depends on your research application, such as antibody production, receptor/ligand studies, or structural studies. Higher purity levels are generally recommended for more sensitive applications.

What is the Maximum Peptide Length you can produce?

We routinely produce peptides of greater than 70 amino acids in length and can synthesize peptides of over 100 amino acids.

Do I need Amino Acid Analysis?

While Amino Acid Analysis is not universally necessary, it offers an additional layer of validation for your custom peptides, ensuring the accuracy of composition and net peptide content.

Do I need Amino Acid Sequencing?

Under solid-phase synthesis, the sequence of peptides is usually not in question. Mass spec analysis often suffices to confirm the synthesis completion. However, sequencing might be recommended for certain peptides like MAPS peptides.

How can I find the Net Peptide Content?

Net peptide content is determined with an Amino Acid Analysis.

What are Peptide Arrays and how can I use them?

Peptide Arrays involve the production of a large number of peptide sequences for use in high throughput screening. They are used for epitope mapping, peptide libraries, protein characterization, and more.

What are Peptide Libraries and how can I use them?

Peptide libraries are systematic combinations of different peptides. They are used for drug discovery, structural studies, and other applications such as antibody specificity variations and bioactive peptide identification.

What is PEGylation and how can I use it?

PEGylation, the strategic attachment of polyethylene glycol to peptides, significantly enhances the stability, bioavailability, and therapeutic efficacy of peptide-based pharmaceuticals.

How is KLH-Peptide conjugation useful?

KLH-Peptide conjugation enhances the efficacy of peptides in therapeutic vaccines. KLH is a potent immunogenic carrier protein used for antibody production.

How is BSA-Peptide conjugation useful?

BSA-Peptide conjugation is used for antibody production. BSA, being highly soluble and immunogenic, is a popular choice for weakly antigenic compounds and immunoassays.

What is the other 10% in a 90% pure peptide?

The other 10% in a 90% pure peptide consists of incomplete or differently sequenced chains. Peptide purity is determined by analyzing and purifying crude peptides to achieve the desired purity.

What is Net Peptide Content?

Net peptide content is the actual percentage weight of peptide in the final product, excluding non-peptide components like water, absorbed solvents, counter ions, and salts.

Peptide Purity vs. Net Peptide Content

Peptide purity quantifies the exact proportion of the target sequence in your sample, whereas net peptide content precisely measures the ratio of peptide to non-peptide components, offering a comprehensive understanding of your peptide's composition.

How should I store my peptides?

Lyophilized peptides should be stored in a dry environment with desiccants, preferably in a freezer at -20 degrees C for optimal shelf life.

Can I store peptides in solutions?

Storing excess peptides in solution is not recommended due to limited shelf life, especially for sequences containing certain amino acids. If necessary, use a sterile buffer at pH 5-6 and store at -20C.

How do I reconstitute my peptides?

Peptides are generally easy to solubilize. Try reconstituting your peptide in a small amount of the intended solvent first. For solubility issues, refer to our Solubility Recommendation.

Why does solubility vary amongst peptides?

Solubility varies due to the unique physical characteristics of each amino acid. The quantity and sequence of amino acids in the peptide determine its solubility.